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Background: The purpose of this study was to determine the effects of whole body vibration (WBV) exercise on lower extremity muscle activity and gait ability in stroke patients. Methods: For this study, 30 stroke patients participated in this study and they were divided into WBV exercise group and sham-WBV exercise group, each group in which consisted of 15 patients. WBV group and sham-WBV group was performed by the patients for five times a week, for six weeks. sEMG was used to measure lower extremity muscle activity. Changes in the activities of the muscles, such as the vatus lateralis (VL), vastus medialis (VM), bicep femoris (BF), gastrocnemius (GCM) muscle, were analysis. Motion analysis system was used to measure gait ability. Gait ability measured the stride length (SL) and walking velocity (WV). Results: According to the results of the comparisons between the groups, after intervention, lower extremity muscle activity of VL (p＜.01), VM (p＜.01), GCM (p＜.01). SL, WV was significant between the group (p＜.01). Conclusion: This study showed the WBV exercise is effective for improving increase of muscle activity and gait ability in stroke patients.