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Background: This study aimed to investigate the effect of hold-relax and antagonist contraction (HR-AC) technique and Kinesio taping on pain changes in delayed-onset muscle soreness (DOMS) of 20`s adults. Methods: Based on voluntary participation, 14 participants with induced-DOMS were randomly divided into control (non-treatment, n=7) and experiment group (HR-AC technique and kinesio taping, n=7). Measurement were used visual analogue scale (VAS), heart rate variability (HRV), and brain wave (alpha). There were 5 sets of the HR-AC technique application on the 5th day from the day of DOMS induction (rest for 30s between sets). Kinesio taping was applied to the same area after HR-AC. Results: The VAS showed significant changes according to the period and in the interaction between the period and the group (p<.01). HRV and alpha wave (Fp1) showed significant changes according to the period (p<.001). The analgesic effect was more rapid and continuous in the experimental group than in the control group. Conclusion: This study showed that the HR-AC technique and Kinesio taping are effective interventions for the management of pain and stress caused by DOMS.