earticle

영어

Polysaccharides from marine algae are known to exhibit various biological and pharmacological activities, including anticancer, immunostimulatory, anti-inflammatory, anti-coagulant, antioxidant, antibacterial, and antiviral activities, offering a variety of potential applications in the fields of foods, cosmetics, drug-delivery, tissue engineering and regenerative medicine, vaccines, and pharmaceutical industries. Sargassum horneri, which is a brown alga and abundantly grows along the coasts of Jeju island in South Korea, has anti-inflammatory, antioxidant and immnomodulatory activities. However, the anticancer effect against human colon cancer has not been revealed yet. We isolated polysaccharides from S. horneri (termed as SHP), analyzed the monosaccharides composition and molecular weight, and investigated the anticancer activity using human colon cancer cell line, HT-29 cells. Bio-LC and HPLC analysis showed that SHP mainly consists of fucose, galactose, and glucose with the molar ratios of 73.37, 17.61 and 9.02 mol%, and has molecular weight of approximately 122,153 and 14,170 kDa. SHP dose-dependently reduced cell viability on HT-29 and increased the cleavage of poly (ADP-ribose) polymerase(PARP) by approximately 10-fold compared to the untreated control group. Furthermore, SHP treatment decreased the procaspase-9, procaspase-8 and Bcl-2 protein expression by about 0.4, 0.7, and 0.3-fold compared to the untreated control group in HT-29 cells. Taken collectively, SHP is a mixture of high-molecular weight glucogalactofucan type polysaccharides and induces apoptotic cell death of human colon cancer cells via upregulation of the expression of apoptosis-related factors, suggesting that S. horneri-derived polysaccharides can be potent ingredients for health-beneficial foods or anti-cancer agents against human colon cancer.