원문정보
초록
영어
endo-(1,5)-α-L-Arabinanase (AN, EC 3.2.1.99) is an endo-acting hydrolase which can randomly hydrolyze the internal α-(1,5)-L-arabinofuranosidic linkages of arabinan polymers. The arabinan-utilization gene cluster was found from the genome of Streptococcus equinus, and a probable extracellular endo-AN GH43 (SteqAN43B) gene was cloned and heterologously expressed in Escherichia coli. The corresponding open reading frame consists of 2,283 nucleotides encoding 761 amino acids. This enzyme possesses the longer N- and C-terminal domains, compared with common bacterial ANs. Although the debranched (linear) arabinan is the most preferred substrate, SteqAN43B showed the significant hydrolytic activity on sugar beet (branched) arabinan as well. Its modes of action are suitable for the production of various branched arabinooligosaccharides from sugar beet arabinan, and the synergistic hydrolysis of arabinans in combination with the other exo-AFs.