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Abstracts for poster Presentation

Functional Expression and Comparative Characterization of Arabinan-Degrading exo-Hydrolases Cloned from Lactobacillus shenzhenensis

초록

영어

From the genome of Lactobacillus shenzhenensis, the probable genes encoding five α -L-arabinofuranosidases (AF, EC3.2.1.55) and an β-L-arabinopyranosidase (AP, EC3.2.1.88) were found and cloned in Escherichia coli. Except LbshAF-B1, five genes were functionally expressed and characterized in this study. The comparative studies on the hydrolytic modes of action revealed that these exo-hydrolases can play the important roles in the enzymatic saccharification of arabinan polymers into L-arabinose. LbshAF-A GH51 could hydrolyze both α-(1,5)- and α-(1,2)/(1,3)-linkages in arabinan. LbshAF-B2 GH51 showed only α-(1,2)/(1,3)-specific debranching activity to generate linear products, whereas LbshAF-C1 and C2 GH43 could exclusively hydrolyze α-(1,5)-linked arabinan backbone. LbshAP showed the activity against p-nitrophenyl arabinopyranoside. It was proposed that the synergistic actions of these exo-hydrolases can facilitate the complete degradation of sugar beet arabinan to L-arabinose.

저자정보

  • Tae Hyeon Park Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea
  • Chang-Yun Choi Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea
  • Jeong-Rok Song Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea
  • Damee Park Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea
  • Tae-Jip Kim Brain Korea 21 Center for Bio-Health Industry, and Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Korea

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