earticle

영어

On-chip glycan biosynthesis is an effective strategy for preparing useful complex glycan sources and for applying glycan-involved applications simultaneously. However, current methods have some limitations of analyzing the biosynthesized glycans and optimizing the enzymatic reactions. This could result in undefined glycan structures on a surface, which leads to unequable and unreliable results. In this work, a novel glycan chip was developed by introducing pH-responsive i-motif DNA linker to control the immobilization and isolation of glycans on chip surface in pH-dependent manner. On-chip enzymatic glycosylations were optimized for uniformly biosynthesizing cancer-associated Globo H hexasaccharide and its related complex glycans through stepwise quantitative analyses of isolated products from the surface. Successful interaction analyses of anti-Globo H antibody and MCF-7 breast cancer cells with on-chip biosynthesized Globo H-related glycans demonstrated the feasibility of the structure switchable DNA linker-based glycan chip platform for on-chip complex glycan biosynthesis and glycan-involved applications.