원문정보
초록
영어
This study investigated the antihypertensive effects of an antioxidant peptide, Leu-Trp-His-Thr-His (LWHTH), purified from Styela clava peptic hydrolysate, to assess the bioactivity of the peptide and verify the value of S. clava as a health-promoting food. Also, the study presented structural evidence for the effects of LWHTH. The inhibitory effect of LWHTH on angiotensin I-converting enzyme (ACE) was assessed using enzyme reaction methods and the simulation methods in computational space. LWHTH inhibited ACE with an IC50 value of 16.42 – 0.45 lM. The LWHTH structure was stable, and its ACE inhibitory effect was retained under simulated gastrointestinal conditions. In silico simulations revealed that LWHTH binds the active site of ACE, with residues LW making the ACE–LWHTH complex stable and residues HTH making the complex strong. Furthermore, LWHTH significantly reduced blood pressure in spontaneously hypertensive rats. These results demonstrate that LWHTH has the potential to be a healthy functional food with antihypertensive effects. Therefore, S. clava consumption may be beneficial for human health.
목차
INTRODUCTION
MATERIALS AND METHODS
Experimental materials
ACE inhibition effect
Stability against gastrointestinal enzymes
In silico docking to ACE
Blood pressure regulation in spontaneouslyhypertensive rats
Statistical analysis
RESULTS
ACE inhibition effect of LWHTH
Molecular docking analysis of LWHTH and ACE
Antihypertensive effect of LWHTH in SHRs
DISCUSSION
AUTHOR DISCLOSURE STATEMENT
FUNDING INFORMATION
REFERENCES