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논문검색

Molecular expression analysis and characterization of rockfish (Sebastes schtegetu) B cell activating factor

목차

Abstract
I. Introduction
2. Materials and methods
2.1. Identification and in-silico analysis of rockfish BAFF cDNA sequence
2.2. Rockfish culturing and tissue collection
2.3. Immune challenge
2.4. RNA extraction and cDNA synthesis
2.5. Quantitative real time PCR (qPCR) analysis
2.6. Cloning and purification of rSsBAFF pretein
2.7. Rockfish lymphocytes isolation
2.8. WST-1 assay
2.9. Flow cytometric analysis
2.10. Analysis of downstream gene expression of S. schlegelii IL-10, NFkB-2. Bax, and Bcl-2
2.11. Statistical analysis
3. Results and discussion
3.1. Sequence characterization of SsBAFF
3.2. 3D modeling of SsBAFF
3.3. Tissue specific transcriptional pattern of SsBAFF
3.4. SsBAFF mRNA expression in immune-challenged spleen
3.5. Functional analysis
4. Conclusion
Declaration of Competing Interest
Acknowledgments
Appendix A. Supplementary data
Referneces

저자정보

  • W.S.P. Madhuranga Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea
  • M.D. Neranjan Tharuka Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea
  • Hyerim Yang Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea
  • Chaehyeon Lim Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea
  • Qiang Wan Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea
  • S.D.N.K. Bathige Sri Lanka Institute of Nanotechnology(SLINTEC), Nanotechnology and Science Park, Mahenwatta, Pitipana, Homagama, Sri Lanka
  • Jehee Lee Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea, Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea

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