원문정보
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Abstract
I. Introduction
2. Materials and methods
2.1. Identification and in-silico analysis of rockfish BAFF cDNA sequence
2.2. Rockfish culturing and tissue collection
2.3. Immune challenge
2.4. RNA extraction and cDNA synthesis
2.5. Quantitative real time PCR (qPCR) analysis
2.6. Cloning and purification of rSsBAFF pretein
2.7. Rockfish lymphocytes isolation
2.8. WST-1 assay
2.9. Flow cytometric analysis
2.10. Analysis of downstream gene expression of S. schlegelii IL-10, NFkB-2. Bax, and Bcl-2
2.11. Statistical analysis
3. Results and discussion
3.1. Sequence characterization of SsBAFF
3.2. 3D modeling of SsBAFF
3.3. Tissue specific transcriptional pattern of SsBAFF
3.4. SsBAFF mRNA expression in immune-challenged spleen
3.5. Functional analysis
4. Conclusion
Declaration of Competing Interest
Acknowledgments
Appendix A. Supplementary data
Referneces
I. Introduction
2. Materials and methods
2.1. Identification and in-silico analysis of rockfish BAFF cDNA sequence
2.2. Rockfish culturing and tissue collection
2.3. Immune challenge
2.4. RNA extraction and cDNA synthesis
2.5. Quantitative real time PCR (qPCR) analysis
2.6. Cloning and purification of rSsBAFF pretein
2.7. Rockfish lymphocytes isolation
2.8. WST-1 assay
2.9. Flow cytometric analysis
2.10. Analysis of downstream gene expression of S. schlegelii IL-10, NFkB-2. Bax, and Bcl-2
2.11. Statistical analysis
3. Results and discussion
3.1. Sequence characterization of SsBAFF
3.2. 3D modeling of SsBAFF
3.3. Tissue specific transcriptional pattern of SsBAFF
3.4. SsBAFF mRNA expression in immune-challenged spleen
3.5. Functional analysis
4. Conclusion
Declaration of Competing Interest
Acknowledgments
Appendix A. Supplementary data
Referneces
저자정보
참고문헌
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