원문정보
초록
영어
Recently, a putative new hyperparasitic haplosporidian in the genus Urosporidium was identified from metacercariae of the trematode Parvatrema duboisi infecting Manila clam Ruditapes philippinarum on the west coast of Korea. In this study, we applied small subunit ribosomal DNA (SSU rDNA) sequences as a marker to substantiate the phylogenetic relationship of the unidentified Urosporidium within the Order Haplosporida. In our phylogenetic analysis, the 1890 bp of SSU rDNA sequences obtained were closely related to a haplosporidian parasite forming a sister clade to Urosporidium group, although the gene sequences were only 89.22–89.70% similar to Urosporidium spp. Such molecular phylogenetic distance within the genus suggested that the unidentified Urosporidium is a new member of the genus. Accordingly, we report the unidentified haplosporidian hyperparasite as Urosporidium tapetis sp. nov.
목차
1. Introduction
2. Materials and methods
2.1. Clam sampling and spore isolation from the host
2.2. Scanning electron microscopy (SEM)
2.3. DNA extraction, amplification, and sequencing of Urosporidium sp.
3. Results and discussion
3.1. Spore isolation and SEM
3.2. DNA sequence and phylogenetic analysis
Acknowledgments
References