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Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme

원문정보

Lei Wang, Thilina U. Jayawardena, Hye-Won Yang, Hyo Geun Lee, Min-Cheol Kang, K. K. Asanka Sanjeewa, Jae Young Oh, You-Jin Jeon

제주대학교 해양과학연구소 해양과학연구소 연구논문집 제44권 2020.12 pp.176-189
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

The previous study suggested that the sulfated polysaccharides from Hizikia fusiforme (HFPS) possess strong antioxidant activity. The purpose of this study is to isolate fucoidan from HFPS and to investigate its antioxidant activity. Afucoidan (HFPS-F4) with a molecular weight of 102.67 kDa was isolated from HFPS. HFPS-F4 contains 99.01% of fucoidan (71.79 ± 0.56% of carbohydrate and 27.22 ± 0.05% of sulfate content). The fucoidan increased the viability of H2O2-treated Vero cells by 5.41, 11.17, and 16.32% at the concentration of 12.5, 25, and 50 μg/mL, respectively. Further results demonstrated that this effect act diminishing apoptosis by scavenging intracellular reactive oxygen species (ROS) via increasing the expression of the endogenous antioxidant enzymes, which was induced by elevating total nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels. In addition, the in vivo test results displayed that the pretreatment of fucoidan improved the survival rates and decreased heart-beating rate, ROS, cell death, and lipid peroxidation in H2O2-stimulated zebrafish. Taken together, these results demonstrated that fucoidan isolated from HFPS has strong in vitro and in vivo antioxidant activities and it could be utilized in pharmaceutical, nutraceutical, and cosmeceutical industries.

목차

Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Chemicals
2.2. Extraction and Isolation
2.3. Free Radical Scavenging Activity
2.4. Chemical Composition Analysis
2.5. Chemical Characterization of HFPS-F4
2.6. Cell Culture
2.7. Effect of Fucoidan on H2O2-Stimulated Vero Cells
2.8. Effect of Fucoidan on H2O2-Stimulated Zebrafish
2.9. Statistical Analysis
3. Results and Discussion
3.1. Free Radical Scavenging Activities of the Polysaccharides Isolated from HFPS
3.2. Chemical Composition and Structural Characteristics of HFPS-F4
3.3. In Vitro Antioxidant Activity of Fucoidan
3.4. In Vivo Antioxidant Activity of Fucoidan
4. Conclusions
References

키워드

저자정보

  • Lei Wang Department of Marine Life Sciences, Jeju National University, Jeju, Marine Science Institute, Jeju National University, Jeju, Jeju Self-Governing Province 63333, Korea
  • Thilina U. Jayawardena Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea
  • Hye-Won Yang Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea
  • Hyo Geun Lee Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea
  • Min-Cheol Kang Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea, Research Group of Process Engineering, Korea Food Research Institute, Wanju, Jeollabuk-do 55365, Korea
  • K. K. Asanka Sanjeewa Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea
  • Jae Young Oh Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Self-Governing Province 63243, Korea
  • You-Jin Jeon Department of Marine Life Sciences, Jeju National University, Jeju, Marine Science Institute, Jeju National University, Jeju, Jeju Self-Governing Province 63333, Korea

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