원문정보
초록
영어
In our previous study, the anticancer effect of the active fucoidan (JHCF4) isolated from acid-processed Sargassum fusiformis was evaluated. In this study, the liver-protective effects of JHCF4 against ethanol-induced Chang liver cell damage and apoptosis-related responses were investigated. Furthermore, the low cytotoxicity and high cell viability of JHCF4 against ethanol-induced cell damage, as well as its protective effect against ethanol-induced cell apoptosis, were observed via nuclear staining with Hoechst 33342 in Chang liver cells. Additionally, the treatment of the 72 h post-fertilization (hpf) zebrafish model with JHCF4 increased the ethanol-stimulated survival rates as well as decreased oxidative stress, lipid peroxidation, and cell death levels. JHCF4 was found to significantly decrease steatosis production in the 128 hpf zebrafish model by Oil Red O staining, as well as attenuate the malondialdehyde and increase the glutathione contents, compared with the untreated group. These results demonstrate that JHCF4 has a potential hepato-protective effect against ethanol-induced damage both in vitro and in vivo.
목차
Introduction
Materials and methods
Extraction and separation of JHCF4 from Sargassumfusiformis
Cell culture
Protective effect of JHCF4 against ethanol-inducedcellular damage
Nuclear staining with Hoechst 33342
Apoptosis analysis by flow cytometry
Western blot analysis
Analysis of hepatic oxidative stress in ethanol-treatedzebrafish embryos (72 hpf)
Measurement of steatosis, malondialdehyde, andglutathione content in ethanol-treated zebrafish larvae(128 hpf)
Results
Separation and chemical composition of JHCF4
Protective effect against ethanol-induced damage inChang liver cells
Protective effect of JHCF4 against ethanol-treatedzebrafish embryos (72 hpf)
Protective effect of JHCF4 against ethanol-treatedzebrafish larvae (128 hpf)
Discussion
Conclusions
Compliance with ethical standards
References