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논문검색

Short Communication

Evaluation of the effect of protease inhibitors on the viability of Miamiensis avidus using the WST-1 assay

초록

영어

Miamiensis avidus causes scuticociliatosis in cultured olive flounders (Paralichthys olivaceus), leading to economic losses in aquaculture in Korea. Quantitative evaluation of the viability of M. avidus is important to develop an effective vaccine or chemotherapeutic agent against it. We used a colorimetric assay based on the reduction of 2- (4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1) to quantify the viability of M. avidus. Using this method, we investigated the effect of protease inhibitors on the viability of M. avidus. The assay showed a clear difference in the optical density (OD) of over 104 ciliates, and the metalloprotease inhibitors 1, 10-phenanthroline and ethylenediaminetetraacetic acid (EDTA) reduced the viability of M. avidus by more than 90% when used at concentration of 5 mM and 100 μM, respectively. However, different morphological changes in the parasite were observed when exposed to these two inhibitors. These results indicate that the WST-1 assay is a simple and reliable method to quantify the viability of M. avidus, and metalloproteases are excellent targets for the development of agents and vaccines to control M. avidus infection.

목차

ABSTRACT
Declaration of Competing Interest
Acknowledgements
References

저자정보

  • Sang Phil Shin Department of Marine Life Science, Jeju National University, Jeju Self-Governing Province, Marine Science Institue, Jeju National University, Jeju Self-Governing Province
  • Han Chang Sohn Department of Marine Life Science, Jeju National University, Jeju Self-Governing Province, Marine Science Institue, Jeju National University, Jeju Self-Governing Province
  • Chang Nam Jin Department of Marine Life Science, Jeju National University, Jeju Self-Governing Province, Marine Science Institue, Jeju National University, Jeju Self-Governing Province
  • Jehee Lee Department of Marine Life Science, Jeju National University, Jeju Self-Governing Province, Marine Science Institue, Jeju National University, Jeju Self-Governing Province

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