원문정보
피인용수 : 0건 (자료제공 : 네이버학술정보)
목차
ABSTRACT
1. Introduction
2. Materials and Methods
2.1 Identification of MyD88 from rockfish and in silico analysis
2.2 Fish rearing
2.3 Tissue/organ sampling for assays of tissue distribution and gene expression upon immune challenge
2.4 Construction of the expression plasmid
2.5 Cell culture and transfection
2.6 Inflammatory gene expression assay
2.7 RNA extraction and cDNA synthesis
2.8 Quantitative real-time PCR(qPCR)
2.9 Luciferase assay
2.10 NO Production and F-action fluorescent detection
2.11 Statistical analysis
3. Results
3.1 In silico analysis of SsMyD88
3.2 Unchallenged spatial expression pattern of SsMyD88 in vivo
3.3 Immune stimulants and temporal fluctuation of SsMyD88 in vivo
3.4 Downstream promoter biases for SsMyD88
3.5 SsMyD88 induces inflammatory activation
4. Discussion
5. Conclusion
Acknowledgments
References
1. Introduction
2. Materials and Methods
2.1 Identification of MyD88 from rockfish and in silico analysis
2.2 Fish rearing
2.3 Tissue/organ sampling for assays of tissue distribution and gene expression upon immune challenge
2.4 Construction of the expression plasmid
2.5 Cell culture and transfection
2.6 Inflammatory gene expression assay
2.7 RNA extraction and cDNA synthesis
2.8 Quantitative real-time PCR(qPCR)
2.9 Luciferase assay
2.10 NO Production and F-action fluorescent detection
2.11 Statistical analysis
3. Results
3.1 In silico analysis of SsMyD88
3.2 Unchallenged spatial expression pattern of SsMyD88 in vivo
3.3 Immune stimulants and temporal fluctuation of SsMyD88 in vivo
3.4 Downstream promoter biases for SsMyD88
3.5 SsMyD88 induces inflammatory activation
4. Discussion
5. Conclusion
Acknowledgments
References
저자정보
참고문헌
자료제공 : 네이버학술정보