원문정보
초록
영어
This study performed molecular genetic identification using DNA barcode markers such as internal transcribed spacer(ITS) marker and nuclear large subunit(nLSU) marker in ribosomal DNA regions on 51 mushroom specimens which were previously classified as poisonous mushrooms according to the morphological identification method in Rural Development Administration(RDA). As a result, nine of the 51 mushroom specimens were identified as different species from the previously identified species, and one of them was identified as edible and medicinal mushroom, not as a poisonous mushroom. Based on the results of this study, it is considered that more accurate species identification results can be obtained by performing molecular genetic identification method using DNA barcode marker as well as morphological identification method in mushroom species identification.
목차
I. 서론
II. 재료 및 방법
1. 독버섯 시료 및 DNA 분리
2. PCR 증폭 및 DNA 염기서열 분석
3. 염기서열 데이터 분석
III. 결과 및 고찰
1. 독버섯 시료에서 ITS 마커와 nLSU 마커의 증폭
2. 독버섯 시료를 대상으로 ITS 마커와 nLSU 마커를 이용한 분자유전학적 분석
IV. 결론
V. 사사
VI. 참고문헌