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Objectives: This study aimed to investigate the effects of self acupressure on eye fatigue and class concentration in college students. Methods: This study was based on the non-equivalent control group pretest-posttest design as a quasi-experimental design. Data were collected from June 8 to July 3, 2020. The experimental group received self-acupressure at specific acupoints (jingming, cuanzhu, sizhukong, tongziliao, and chengqi) thrice a day for 5 days, followed by a 2-day rest period. The control group was administered artificial tears for 4 weeks. Sixty women were divided into the experimental and control groups (n = 30 in each). The collected data were analyzed by chi-square test, paired t-test, and independent t-tests using the Statistical Package for the Social Sciences version 21.0 program. Results: There was a significant decrease in eye fatigue (t = −8.85, p = <.001 ) and an increase in class concentration (t = 7.79, p = <.001) in the experimental group as compared to the control group in the pretest-posttest evaluation. Conclusions: The findings confirmed that self-acupressure is an effective nursing intervention to not only decrease eye fatigue experienced by college students, but also increase their class concentration.