원문정보
초록
영어
As the importance of biological resources increases, the conservation technology is becoming important for rarities. This study was conducted to establish an efficient cryopreservation conditions for the Deutzia paniculata, endangered plant species, by using both cryopreservation methods of vitrification and encapsulation. As a result, the sucrose pretreatment seed viability showed up to 30.7% in the treatments. The cryoprotectant treatment improved the viability of the seeds, and was found to be excellent in the vitrification method using PVS3. The vitrification method had over 10% higher germination rate than the seeds preserved by encapsulation. In addition, the germination rate showed a significant difference according to the cryopreservation treatment time, and the germination rate of seeds decreased very much as the long time became longer. Plants germinated from preserved seed in liquid nitrogen showed poor growth compared to untreated, and good growth in PVS3 120 minutes. In addition, the growth of germinated plants by liquid nitrogen treatment time was better in the vitrification method. These results are expected to be useful for long-term preservation of D. paniculata, endangered plants.
목차
Introduction
Materials and Methods
Plant materials and cultivation
Sucrose pretreatment
Encapsulation method
Vitrification method
Thawing and plant formation
Measurement of seed viability
Measurement of germination rate
Growth and acclimation of the in vitro seedlings
Statistical analysis
Results
Seed viability by sucrose pretreatment
Germination rate by cryopreservation method
Acclimatization and growth of seedlings cryopreserved using LN
Discussion
References