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Purpose: This study aimed to identify influencing factors on the stress of employees in large corporations by examining relationships between self-esteem, emotional intelligence, and mindfulness. Methods: A hundred and sixty four employees from large corporations completed self-reporting questionnaires during November and December, 2019. The collected data were analyzed with SPSS/WIN 22.0 using descriptive statistics, the t-test, ANOVA, correlation, and multiple regression analysis. Results: The scores were 2.96±0.40 for “self-esteem”, 3.56±0.61 for “emotional intelligence”, 4.01±0.04 for “mindfulness”, and 2.21±0.79 for “stress response”. A regression analysis identifying factors affecting the stress response of employees in large corporations showed that “mindfulness” was the most significant factor (β=-.58, p<.001), followed by “emotional intelligence” (β=-.21, p=.005) and “self-esteem” (β=-.16, p=.009). The explanatory power of these variables for the stress response of employees in large corporations was 48%. Conclusion: Our findings suggest that self-esteem, emotional intelligence, and mindfulness are significantly related to employees’ stress response in large corporations. Therefore, stress response management programs should be developed to improve self-esteem, emotional intelligence, and mindfulness.