원문정보
한국동물생명공학회(구 한국동물번식학회)
Journal of Animal Reproduction and Biotechnology
Volume. 35 No. 3
2020.09
pp.268-272
피인용수 : 0건 (자료제공 : 네이버학술정보)
초록
영어
Amniotic membrane stem cells are considered as a good alternative to embryonic stem cells, but their use in clinical studies is still not common. Here, exosomes from canine amniotic membrane mesenchymal stem cells (cAmMSCexo) were used for dog sperm cryopreservation. Upon cryopreserved straws using cryoprotectant containing 0, 0.5, 1, or 2 μg/mL of cAmMSC-exo were thawed, motility and membrane integrity were analyzed. However, results showed no significant differences between the groups. We concluded that cAmMSC-exo with lower than 2 µg/mL have no effects on sperm cryopreservation, and further studies to get higher concentrations of cAmMSC-exo should be conducted for clinical application.
목차
ABSTRACT
INTRODUCTION
MATERIEL AND METHODS
Cell culture and exosomes extraction
Animal use and semen processing
Cryopreservation and thawing process
Sperm parameters evaluation
RESULTS AND DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES
INTRODUCTION
MATERIEL AND METHODS
Cell culture and exosomes extraction
Animal use and semen processing
Cryopreservation and thawing process
Sperm parameters evaluation
RESULTS AND DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES
저자정보
참고문헌
자료제공 : 네이버학술정보