earticle

영어

The range of possible growth temperature of the Lentinula edodes mycelium is 5~32℃, and the appropriate temperature is 22~26℃, which is generally known as medium and high temperature. In order to check the expression level of the gene according to the temperature during growth, the temperature was divided into three treatments of 10, 20, and 30℃ to grow the altitude, and 20℃ was used as a control. The result is as follows. a total of 32,706 genes were identified, and 1319 genes were identified at 10℃ compared to the control for those with a more than 2-fold change expression level. among them, 591 genes were up-regulated at 10℃ compared to the control. it was confirmed that 728 genes were down-regulated, and those grown at 30°C were 6,977 genes that expressed more than 2 fold change compared to the control, and the number of up-regulated genes was 3,703 down-regulated. 3,274 genes were identified. among the upregulated genes, cytochrome P450, Alpha beta hydrolase fold protein, chondroitin AC alginate lyase, and proline-specific peptidase were identified. Among the downregulated genes, farnesyl-diphosphate synthase, zinc finger transcription factor 1, and glycolytic enzyme family 79 proteins were identified.