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Objectives: This study was conducted to identify factors influencing the performance of multidrug-resistant organism (MDROs) infection control in general hospital nurses. Methods: Data were collected from August 5 to August 16, 2019. Participants were made up of 179 nurses. Descriptive statistics, t-test, ANOVA, Pearson correlation coefficients and multiple regression were used to analyze the data. Results: Multiple regression analysis showed that performance with Methcillin resistant staphylococcus aureus(MRSA) infection control was significantly affected by organizational culture(β=.255, p= .001), and performance with Vancomycin resistant enterococcus(VRE) and Carbapenem Resistant gram-negative bacteria(CRGNB) infection control were significantly affected by knowledge((β=0.327, p<.001, β=0.183, p<.019) and, organizational culture(β=0.313, p<.001, β=0.293, p<.001). Conclusions: The results of this study indicate that the organizational culture and knowledge were highly influential factors affecting compliance of MDROs infection control in general hospital nurses. Therefore, in order to improve the nurses’ performance for the MDROs infection control, it was necessary to create positive organizational culture and develop practical ongoing training or education programs.