earticle

영어

Purpose: This study is to evaluate biological activity of leaf and seed extracts of Brassica juncea L and examine their utility value as cosmetic ingredients. Methods: Total polyphenol, total flavonoid content, DPPH radical scavenging activity, ABTS radical scavenging activity, and inhibition activities of tyrosinase and elastase were measured to evaluate biological activity of leaf and seed extracts of Brassica juncea L. Czern.(EtOH). In addition, to investigate the antioxidant effect and enzyme inhibition activities in relation to concentrations, correlation of different concentrations of 50, 100, and 200 mg/mL of each extract were comparatively analyzed. Results: The highest total polyphenol contents of leaf and seed extracts of Brassica juncea L (50, 100, and 200 mg/mL) were 15.03 and 27.72 mg TAE/g, respectively, while the total flavonoid contents were 58.84 and 126.75 mg QE/g, respectively. The DPPH scavenging effect was confirmed as 65.26% in leaf extracts and 83.17% in seed extracts, while the ABTS radical scavenging effect was confirmed as 47.06% in leaf extracts and 60.57% in seed extracts. As a result of evaluation of biological activity analysis on antioxidant effect, the activity in seed was higher than the activity in leaf, and a positive correlation was indicated as highest activities were shown in the concentration of 200 mg/mL for both leaf and seed. The highest inhibition activities of tyrosinase were 22.33% and 36.58%, respectively, while the inhibition activities of elastase were 33.45% and 40.21%, respectively. In leaf extracts, the inhibition activities of tyrosinase and elastase increased as the concentration increased, while in seed extract, the inhibition activities of elastase increased as the concentration increased, but the inhibition activities decreased when the concentration of the extract reached above a certain level. Conclusion: The results of the study confirmed leaf and seed extracts of Brassica juncea L. Czern. have excellent antioxidant effect and inhibition effect on activities of tyrosinase and elastase, and their utility value as cosmetic ingredients would be high.

한국어

목적: 본 연구는 갓의 잎과 종자 추출물의 생리활성 평가를 실시하여 화장품 소재로써 이용가치를 규명하고자 하였다. 방법: 생리 활성 평가를 위해 갓의 잎, 종자 추출물(EtOH)의 총 폴리페놀, 총 플라보노이드 함량, DPPH radical 소거능, ABTS radical 소거능, tyrosinase 저해활성, elastase 저해활성을 측정하였다. 또한 농도에 따른 항산화 효능 및 효소 저해활성을 알아보기 위해 각 추출물 의 농도(50, 100, 200 mg/mL)를 다르게 하여 상관관계를 비교분석 하였다. 결과: 갓의 잎, 종자 추출물(50, 100, 200 mg/mL)의 총 폴리페놀 함량의 최대치는 각각 15.03 mg TAE/g, 27.72 mg TAE/g, 총 플라보노이드 함량은 58.84 mg QE/g, 126.75 mg QE/ g, DPPH radical 소거능은 65.26%, 83.17%, ABTS radical 소거능은 47.06%, 60.57%로 확인되었다. 항산화 관련 생리활성 평가 결 과, 대체로 seed가 leaf 보다 활성이 높았으며, 모두 200 mg/mL 농도에서 가장 높은 결과를 보여 양의 상관관계임을 알 수 있었다. Tyrosinase 저해활성의 최대치는 각각 22.33%, 36.58%, elastase 저해활성은 33.45%, 40.21%였다. Leaf의 경우 농도 의존적으로 tyrosinase 저해활성과 elastase 저해활성이 높아진 반면, seed의 경우 e lastase 저해활성에서는 농도 의존적으로 활성이 높아졌으나 tyrosinase 저해활성에서는 추출물의 농도가 일정수준 이상이 되면 오히려 활성이 낮아지는 것으로 확인되었다. 결론: 본 연구 결과 로 갓(Brassica juncea L. Czern.)의 잎과 종자 추출물은 항산화 효능, tyrosinase 및 elastase 활성 억제 효능의 우수성을 규명할 수 있었으며, 향후 화장품 소재로써 그 이용가치가 높을 것으로 판단된다

중국어

目的: 评估芥菜叶和种子提取物的生物活性，并检验其作为化妆品成分的实用价值。方法: 测定总多酚，总黄酮 含量，DPPH自由基清除活性，ABTS自由基清除活性以及酪氨酸酶和弹性蛋白酶的抑制活性，以评价芥菜叶和 种子提取物的生物活性。另外，为了研究抗氧化剂作用和酶抑制活性与浓度的关系，比较分析了每种提取物的 50、100和200 mg/mL不同浓度的相关性。结果: 芥菜叶和种子提取物中总多酚含量最高（分别为50、100和 200 mg/mL），分别为15.03和27.72 mg TAE/g，而总黄酮含量分别为58.84和126.75 mg QE/g。在叶提取物 中证实了DPPH清除作用为65.26％，在种子提取物中证实为83.17％，而在叶提取物中证实了ABTS自由基清除 作用为47.06％，在种子萃取物中为60.57％。生物活性分析对抗氧化作用的评价结果是，种子中的活性高于叶 中的活性，并且表明正相关性，因为对于叶和种子，在200mg/mL的浓度下，显示出最高的活性。酪氨酸酶的 最高抑制活性分别为22.33％和36.58％，而弹性蛋白酶的抑制活性分别为33.45％和40.21％。在叶提取物中， 酪氨酸酶和弹性蛋白酶的抑制活性随浓度的增加而增加，而在种子提取物中，弹性蛋白酶的抑制活性随浓度的 增加而增加，但当提取物的浓度达到一定水平以上时，其抑制活性降低。结论: 研究结果证实了芸苔属芥子的叶 和种子提取物。对酪氨酸酶和弹性蛋白酶的活性具有极好的抗氧化作用和抑制作用，它们作为化妆品成分的实 用价值很高。