원문정보
초록
영어
Red rose petals are usually disposed but they are an abundant source of phenolics and traditionally used as food supplement and as herbal medicine. Of the Various phenolics, they are known to have anticancer, antioxidant, and anti-inflammatory properties. In this study, we investigated the anti-inflammatory effects of red rose ethanolic extracts (GRP) on lipopolysaccharide (LPS)-activated RAW 264.7 cells. The results demonstrated that pretreatment of GRP (500μg/mL) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects by red rose petals were observed in the following. Red rose petals inhibited the translocation of NF-κB from the cytosol to the nucleus via the suppression of IκB-α phosphorylation and also inhibited LPS-stimulated NF-κB transcriptional activity. These findings suggest that red rose petals exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of red rose petals. Therefore, red rose petals could be regarded as a potential source of natural anti-inflammatory agents.
목차
1. Introduction
2. Experiment Materials and Methods
2.1 Sample Preparation
2.2 Reagent
2.3 DPPH Radical Scavenging Activity
2.4 ABTS Radical Scavenging Activity
2.5 Cell culture
2.6 Nitric oxide & MTT cell viability assays
2.7 Reverse-transcriptase polymerase chain reaction (RT-PCR)
2.8 Statistical analysis
3. Result and Discussion
3.1 Radical scavening activity(DPPH, ABTS) of red rose petal ethanol extracts
3.2 Nitric oxide (NO) production inhibitory effect and cytotoxicity of red rose petal extract
3.3 Measurement of inflammatory effect and cytokine expression levels in Raw264.7 cells
3.4 NO production inhibition and inflammatory mediator and cytokine expression level measurement in MH-S cell
3.5 Pro-inflammatory mediator and cytokine expression in MH-S cells using RT-PCR
4. Conclusion
References