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Background: The purpose of this research is to investigate the effects of joint mobilization and transcutaneous electrical nerve stimulation (TENS) on pain threshold and hormonal changes in patients with chronic LBP. Methods: 14 patients with chronic LBP were divided into the experimental group and the control group with 7 patients each, and both groups of patients were evaluated on pain duration, blood tests, and pressure pain threshold (PPT) on their first visit. On their second visit, joint mobilization was applied to the experimental group for 10 min and TENS to the control group for 20 min. Results: Pain threshold and duration of analgesia increased from after treatment in the experimental group. Serotonin and Cortisol decreased after treatment in the experimental group. Conclusion: Joint mobilization in patients with chronic LBP caused an increase in pain duration and pain thresholds and a decrease in serotonin, whereas there was little difference in cortisol.