원문정보
초록
영어
A high-cholesterol diet can reduce male fertility. However, it is not known whether a high-cholesterol diet can regulate the expression of genes involved in sperm maturation and sperm fertilizing ability. Quercetin, a natural product, is known to have cytoprotective effects by regulating lipid metabolism in various cell types. This study aimed to confirm the expression of genes involved in sperm maturation in the testes of mice fed a high-cholesterol diet and to determine whether quercetin can reverse the genetic regulation of cholesterol. Mice were divided into groups fed a normal chow diet and a high-cholesterol diet. Mice fed the high-cholesterol diet were dose-dependently supplemented with quercetin for 6 weeks. Investigations using quantitative PCR and in situ hybridization revealed that the high-cholesterol diet alters the expression of genes associated with sperm maturation in the testes of mice, and this was reversed with the supplementation of quercetin. In addition, the high-cholesterol diet regulated the expression of genes related to lipid metabolism in the liver of mice. Under a high-cholesterol diet, quercetin can improve male fertility by regulating the expression of genes involved in sperm maturation.
목차
INTRODUCTION
MATERIALS AND METHODS
Experimental animals and diets
Tissue samples
Quantitative RT-PCR analysis
In situ hybridization analysis
Statistical analyses
RESULTS
Effect of quercetin supplementation on high-cholesterol diet-altered expression of Spam1
Effect of quercetin supplementation on high-cholesterol diet-altered expression of Adam5 and Adam29
Effect of quercetin supplementation on high-cholesterol diet-altered expression of Srd5a2 and Srd5a3
Expression of genes involved in lipid metabolism with the high-cholesterol diet and quercetin supplementation
DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES