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Review Article

유전자 편집 기술에 의한 형질전환 가축의 생산 현황

원문정보

Current Status of Production of Transgenic Livestock by Genome Editing Technology

박다솜, 김소섭, 구덕본, 강만종

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The Transgenic livestock can be useful for the production of disease-resistant animals, pigs for xenotranplantation, animal bioreactor for therapeutic recombinant proteins and disease model animals. Previously, conventional methods without using artificial nuclease-dependent DNA cleavage system were used to produce such transgenic livestock, but their efficiency is known to be low. In the last decade, the development of artificial nucleases such as zinc-finger necleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas has led to more efficient production of knock-out and knock-in transgenic livestock. However, production of knock-in livestock is poor. In mouse, genetically modified mice are produced by co-injecting a pair of knock-in vector, which is a donor DNA, with a artificial nuclease in a pronuclear fertilized egg, but not in livestock. Gene targeting efficiency has been increased with the use of artificial nucleases, but the knock-in efficiency is still low in livestock. In many research now, somatic cell nuclear transfer (SCNT) methods used after selection of cell transfected with artificial nuclease for production of transgenic livestock. In particular, it is necessary to develop a system capable of producing transgenic livestock more efficiently by co-injection of artificial nuclease and knock-in vectors into fertilized eggs.

목차

ABSTRACT
서론
유전자 가위(Programmable Nuclease)와 유전자 적중
유전자 가위를 이용한 유전자 편집에 의한 가축 생산
결론과 향후방향(미래 예측)
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES

저자정보

  • 박다솜 Da Som Park. 전남대학교 농업생명과학대학 동물자원학부
  • 김소섭 Soseob Kim. 광개토한우
  • 구덕본 Deog-Bon Koo. 대구대학교 공과대학 생명공학과
  • 강만종 Man-Jong Kang. 전남대학교 농업생명과학대학 동물자원학부

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