원문정보
초록
영어
Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals
Oocytes collection and in-vitro maturation (IVM)
Preparation of donor cells
Generation of somatic cell nuclear transfer (SCNT) embryos
In-vitro culture (IVC) medium used for culturing SCNT embryos
Production of SCNT blastocysts
Preparation of MEF feeder cells
In-vitro outgrowth of inner cell mass (ICM) of SCNT blastocysts
Statistical analysis
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
ORCID
REFERENCES