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Objectives: The purpose of this study was to identify the influences of nursing professionalism and emotional intelligence on the clinical performance ability of nursing students. Methods: The subjects of this study included 234 nursing students from three universities located in two regions of South Korea. Data were collected from June 15 to 30, 2018 by using self-reported questionnaires. The data were analyzed using descriptive statistics, independent t-test, one-way ANOVA, Pearson’s correlation coefficients, and stepwise multiple regression analysis using the IBM SPSS 21.0 program. Results: The factors which influenced the clinical performance ability in nursing students were self-concept of the profession(β=.28, p<.001), roles of nursing service (β=.22, p<.001), use of emotion (β=.22, p<.001), self-emotional appraisal (β=.18, p=.002), regulation of emotion (β=.16, p=.004), and grade (β=.16, p=.002) which explained 51.7% of the variance. Conclusions: These results suggest that to improve clinical performance ability in nursing students, an intervention that enhances their nursing professionalism and emotional intelligence should be considered.