원문정보
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ABSTRACT
1. Introduction
2. Materials and methods
2.1. Identification and bioinformatic analysis of Grx2 sequence from Hippocampus abdominalis
2.2. Acclimatization of big-belly seahorses, tissue sampling and immune stimulation
2.3. RNA isolation and cDNA synthesis
2.4. mRNA expression profiling by quantitative real-time PCR (qPCR) analysis
2.5. Recombinant HaGrx2-pMALc5X plasmid construction
2.6. Overexpression and purification of recombinant HaGrx2
2.7. Cell culture and transfection of HaGrx2
2.8 Functional assays
3. Results
3.1. Bioinformatic analysis of HaGrx2
3.2. Spatial and temporal expression profile of HaGrx2
3.3. Overexpression and purification of rHaGrx2 as MBP fusion protein
3.4. Functional studies
4. Discussion
Acknowledgments
References
1. Introduction
2. Materials and methods
2.1. Identification and bioinformatic analysis of Grx2 sequence from Hippocampus abdominalis
2.2. Acclimatization of big-belly seahorses, tissue sampling and immune stimulation
2.3. RNA isolation and cDNA synthesis
2.4. mRNA expression profiling by quantitative real-time PCR (qPCR) analysis
2.5. Recombinant HaGrx2-pMALc5X plasmid construction
2.6. Overexpression and purification of recombinant HaGrx2
2.7. Cell culture and transfection of HaGrx2
2.8 Functional assays
3. Results
3.1. Bioinformatic analysis of HaGrx2
3.2. Spatial and temporal expression profile of HaGrx2
3.3. Overexpression and purification of rHaGrx2 as MBP fusion protein
3.4. Functional studies
4. Discussion
Acknowledgments
References
저자정보
참고문헌
자료제공 : 네이버학술정보