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Carbohydrate Polymers

Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-kB signal pathway

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ABSTRACT
1. Introduction
2. Martials and methods
2.1. Chemicals and regents
2.2. Collection and preparation of crude polysaccharide sample
2.3. Separation of fucoidan anion-exchange chromatography
2.4. FT-IR characterization, NMR analysis, and quantification of neutral sugar composition of polysaccharide fractions
2.5. Determination of the sulfate content
2.6. Cell culture
2.7. Cell viability and nitric oxide release evaluation assay
2.8. Nuclear and cytosolic protein fractionation
2.9. Westem blotting
2.10. RNA extraction and gene expression analysis by RT-qPCR
2.11. Statistical analysis
3. Results
3.1. Yield and chemical composition of seaweed extract, crude polysaccharide, and isolated fractions
3.2. FT-IR spectrums analysis of isolated polysaccharide fractions
3.3. 1H NMR and monosaccharide composition of active fraction
3.4. Cyto-toxicity and NO inhibition properties of PCCP
3.5. DEAE-cellulose colunm fractions of PCCP reduced LPS-induced NO production and cytotoxicity
3.6. The inhibitory effect of f7 against iNOS and COX2 protein and gene expression from LPS-exposed macrophages
3.7. f7 inhibit LPS-activated pro-inflammatory cytokine mRNA expression from RAW 264.7 cells
3.8. f7 inhibit LPS-activated TLR-2 and TLR-4 mRNA expression from RAW 264.7 cells
3.9. f7 inhibit MyD88 dependent NF-κB activation and translocation to the nucleus
4. Discussion
5. Conclusions
Declaration of Competing Interest
Acknowledgements
References

저자정보

  • K.K. Asanka Sanjeewa Department of Marine Life Science, Jeju National University
  • Thilina U. Jayawardena Department of Marine Life Science, Jeju National University
  • Hyun-Soo Kim Department of Marine Life Science, Jeju National University
  • Seo-Young Kim Department of Marine Life Science, Jeju National University
  • I.P. Shanura Fernando Department of Marine Life Science, Jeju National University
  • Lei Wang Department of Marine Life Science, Jeju National University, Marine Science lnstitute, Jeju National University, Jeju Self-Governing Province 63333, Republic of Korea
  • D.T.U Abetunga Department of Chemistry, University of Colombo, Colombo 3, Sri Lanka
  • Won-Suck Kim College of Medical and Life Sciences, Silla University, Busan, 46958, Republic of Korea
  • Dae-Sung Lee Department of Applied Research, National Marine Biodiversity Institute of Korea, Seocheon, Republic of Korea
  • You-Jin Jeon Department of Marine Life Science, Jeju National University, Marine Science lnstitute, Jeju National University, Jeju Self-Governing Province 63333, Republic of Korea

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