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ABSTRACT
1. Introduction
2. Methodology
2.1. cDNA library construction
2.2. SsGal8 sequence identification and characterization
2.3. Experimental fish and tissue collection
2.4. Immune challenge experiment
2.5. RNA extraction and cDNA synthesis
2.6. SsGal8 expression analysis by qPCR
2.7. Cloning, overexpression and purification of recombinant SsGal8(rSsGal8)
2.8. Hemagglutination assay for rSsGal8
2.9. Sugar specificity assay
2.10. Bacterial agglutination assay
3. Results
3.1. Characterization of SsGal8
3.2. Homology analysis of SsGal8 with other galectin-8 similitudes
3.3. Evolutionary relationships of SsGal8
3.4. Genome structure organization of SsGal8
3.5. Tissue-specific mRNA expression of SsGal8
3.6. Transcriptional modulation of SsGal8 gene expression under immune challenge
3.7. Overexpression and purification of rSsGal8
3.8. Hemagglutination assay and sugar binding assay
3.9. Bacterial agglutination assay
4. Discussion
5. Conclusion
Acknowledgements
Appendix A. Supplementary data
References
1. Introduction
2. Methodology
2.1. cDNA library construction
2.2. SsGal8 sequence identification and characterization
2.3. Experimental fish and tissue collection
2.4. Immune challenge experiment
2.5. RNA extraction and cDNA synthesis
2.6. SsGal8 expression analysis by qPCR
2.7. Cloning, overexpression and purification of recombinant SsGal8(rSsGal8)
2.8. Hemagglutination assay for rSsGal8
2.9. Sugar specificity assay
2.10. Bacterial agglutination assay
3. Results
3.1. Characterization of SsGal8
3.2. Homology analysis of SsGal8 with other galectin-8 similitudes
3.3. Evolutionary relationships of SsGal8
3.4. Genome structure organization of SsGal8
3.5. Tissue-specific mRNA expression of SsGal8
3.6. Transcriptional modulation of SsGal8 gene expression under immune challenge
3.7. Overexpression and purification of rSsGal8
3.8. Hemagglutination assay and sugar binding assay
3.9. Bacterial agglutination assay
4. Discussion
5. Conclusion
Acknowledgements
Appendix A. Supplementary data
References
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