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Life Sciences

A keratinocyte and integrated fibroblast culture model for studying particulate matter-induced skin lesions and therapeutic intervention of fucosterol

원문정보

I.P. Shanura Femando, Thilina U. Jayawardena, Hyun-Soo Kim, A.P.J.P. Vaas, H.I.C. De Silva, C.M. Nanayakkara, D.T.U. Abeytunga, WonWoo Lee, Ginnae Ahn, Dae-Sung Lee, In-Kyu Yeo, You-Jin Jeon

제주대학교 해양과학연구소 해양과학연구소 연구논문집 제43권 2019.12 pp.94-102

목차

ABSTRACT
1. Introduction
2. Materials and methods
2.1. Characterization of CPM
2.2. Purification of fucosterol from Sargasswn binderi
2.3. Evaluation of inhibitory effects of fucosterol on elastase and collagenase
2.4. Cell lines
2.5. The culture of HaCaT and HDF cells
2.6. Cell viability and ROS
2.7. Evaluation of nuclear morphology
2.8. Western blot analysis
2.9. Analysis of cellular collagenase and elastase activity and MMP levels
2.10. Statistical analysis
3. Results
3.1. Size and composition of CPM
3.2. Protective effects of fucosterol against CPM-induced ROS production and apoptotic body formation
3.3. Protective effects of fucosterol against CPM- induced MMP production
3.4. Anti-inflammatory functionalities of fucosterol in HaCaT cells
3.5. Effect of preconditioned HaCaT cell culture media on HDF cell inflammatory responses
4. Discussions
5. Conclusions
Acknowledgments
References

키워드

저자정보

  • I.P. Shanura Femando Department of Marine Life Sciences, jeju National University, Department of Marine Bio-Food Scrences, Chonnam National University
  • Thilina U. Jayawardena Department of Marine Life Sciences, jeju National University
  • Hyun-Soo Kim Department of Marine Life Sciences, jeju National University
  • A.P.J.P. Vaas Department of Chemistry, University of Colombo, Colombo 3, Sri Lanka
  • H.I.C. De Silva Department of Chemistry, University of Colombo, Colombo 3, Sri Lanka
  • C.M. Nanayakkara Department of Plant Sciences, University of Colombo, Colombo 3, Sri Lanka
  • D.T.U. Abeytunga Department of Chemistry, University of Colombo, Colombo 3, Sri Lanka
  • WonWoo Lee Department of Marine Life Sciences, jeju National University, Freshwater Bioresources Utilization Division, Nakdonggang National lnstitute of Biological Resources
  • Ginnae Ahn Department of Marine Bio-Food Scrences, Chonnam National University
  • Dae-Sung Lee Department of Applied Research, National Marine Biodiversity lnstitute of Korea
  • In-Kyu Yeo Department of Marine Life Sciences, jeju National University
  • You-Jin Jeon Department of Marine Life Science, Jeju National University, Marine Science lnstitute, Jeju National University, Jeju Self-Governing Province 63333, Republic of Korea

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