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ABSTRACT
1. Introduction
2. Materials and methods
2.1. Identification of coding sequence of SsVip
2.2. In silico analysis
2.3. Rearing fish
2.4. Challenge experiment and tissue sampling
2.5. Construction of expression plasmids
2.6. Cell culture, VHSV
2.7. Plasmid transfection and virus infection assay
2.8. Total RNA extraction and cDNA synthesis
2.9. Quantitative real-time PCR (qPCR)
2.10 Cell viability analysis through MTT assay
2.11. Subcellular localization
2.12. Statistical analysis
2.13. Key resources table
3. Results and discussion
3.1. In silico analysis of Ss Vip
3.2. Quantitative real time PCR
3.3. Virus transcription reduction by Ss Vip
3.4. Virus replication reduction by Ss Vip
3.5. Subcellular localization of Ss Vip
4. Conclusion
Acknowledgments
Appendix A. Supplementary data
References
1. Introduction
2. Materials and methods
2.1. Identification of coding sequence of SsVip
2.2. In silico analysis
2.3. Rearing fish
2.4. Challenge experiment and tissue sampling
2.5. Construction of expression plasmids
2.6. Cell culture, VHSV
2.7. Plasmid transfection and virus infection assay
2.8. Total RNA extraction and cDNA synthesis
2.9. Quantitative real-time PCR (qPCR)
2.10 Cell viability analysis through MTT assay
2.11. Subcellular localization
2.12. Statistical analysis
2.13. Key resources table
3. Results and discussion
3.1. In silico analysis of Ss Vip
3.2. Quantitative real time PCR
3.3. Virus transcription reduction by Ss Vip
3.4. Virus replication reduction by Ss Vip
3.5. Subcellular localization of Ss Vip
4. Conclusion
Acknowledgments
Appendix A. Supplementary data
References
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