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논문검색

Fish and Shellfish Immunology

Molecular characterization and functional analysis of glutathione S-transferase kappa 1 (GSTκ1) from the big belly seahorse (Hippocampus abdominalis) : Elucidation of its involvement in innate immune responses

원문정보

Anushka Vidurangi Samaraweera, W.M Gayashani Sandamalika, D.S. Liyanage, Sukkyoung Lee, Thanthrige Thiunuwan Priyathilaka, Jehee Lee

제주대학교 해양과학연구소 해양과학연구소 연구논문집 제43권 2019.12 pp.71-81
피인용수 : 0건 (자료제공 : 네이버학술정보)

목차

ABSTRACT
1. Introduction
2. Materials and methods
2.1. Experimental seahorses and tissue isolation
2.2. Challenge experiment
2.3. RNA extraction and cDNA synthesis
2.4. Identification and bioinformatic analysis of HaG5Tk1
2.5. Analysis of mRNA expression of HaGSTk1
2.6. Cloning of HaGSTk1 into pMAL-c5X
2.7. Overexpression and purification of recombinant HaGSTk1(rHaGSTk1) fusion protein
2.8. Functional characterizations of rHaGSTk1
2.9. Statistical analysis
3. Results
3.1. In silico analysis of HaGSTk1
3.2. Tissue distribution of HaGSTk1
3.3. Expression profile of HaGSTk1 mRNA after immune challenges
3.4. Recombinant proteín expression and purification
4. Discussion
Acknowledgments
Appendix A. Supplementary data
References

키워드

저자정보

  • Anushka Vidurangi Samaraweera Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University
  • W.M Gayashani Sandamalika Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University
  • D.S. Liyanage Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Marine Science Institute, Jeju National University, Jeju Self-Goveming Province, 63333, Republic of Korea
  • Sukkyoung Lee Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Marine Science Institute, Jeju National University, Jeju Self-Goveming Province, 63333, Republic of Korea
  • Thanthrige Thiunuwan Priyathilaka Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Marine Science Institute, Jeju National University, Jeju Self-Goveming Province, 63333, Republic of Korea
  • Jehee Lee Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Marine Science Institute, Jeju National University, Jeju Self-Goveming Province, 63333, Republic of Korea

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