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Protective effect of green tea catechin against urban fine dust particleinduced skin aging by regulation of NF-kB, AP-1, and MAPKs signaling pathways

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ABSTRACT
1. Introduction
2. Materials and methods
2.1. Chemicals and reagents
2.2. Cell culture
2.3. Determination of the damage of FDP-stimulated HDFs
2.4. Determination of cytotoxicity of EGCG on HDFs
2.5. Determination of the effect of EGCG against FDP-stimulated HDFs
2.6. Determination of relative intracellular elastase and collagenase activities
2.7. Determination of collagen synthesis and MMPs expression levels by ELISA
2.8. Western blot analysis
2.9. Statistical analysis
3. Results and discussion
3.1. FDP causes oxidative damage and EGCG improves FDP-induced oxidative damage in HDFs
3.2. EGCG inhibits intracellular enzymes activities, protects collagen synthesis, and reduces MMPs expression in FDP-stimulated HDFs
3.3. EGCG inhibits the NF-kB activation, reduces AP-1 phosphorylation, and suppresses MAPKs activation in FDP-stimulated HDFs
4. Conclusion
Conflicts of interest
Acknowledgements
References

저자정보

  • Lei Wang Department of Marine Life Sciences, jeju National University, Marine Science lnstitute, Jeju National University,
  • WonWoo Lee Freshwater Bioresources Utilization Division, Nakdonggang Nationallnstitute of Biological Resources, Sangju 37242 , Republic of Korea
  • Yong Ri Cui Department of Marine Life Sciences, jeju National University
  • Ginnae Ahn Department of Marine Bio Food Science, Chonnam National University, Yeosu, 59626, Republic of Korea
  • You-Jin Jeon Department of Marine Life Sciences, jeju National University, Marine Science lnstitute, Jeju National University

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