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ABSTRACT
1. Introduction
2. Materials and methods
2.1. Chemicals and reagents
2.2. Cell culture
2.3. Determination of the damage of FDP-stimulated HDFs
2.4. Determination of cytotoxicity of EGCG on HDFs
2.5. Determination of the effect of EGCG against FDP-stimulated HDFs
2.6. Determination of relative intracellular elastase and collagenase activities
2.7. Determination of collagen synthesis and MMPs expression levels by ELISA
2.8. Western blot analysis
2.9. Statistical analysis
3. Results and discussion
3.1. FDP causes oxidative damage and EGCG improves FDP-induced oxidative damage in HDFs
3.2. EGCG inhibits intracellular enzymes activities, protects collagen synthesis, and reduces MMPs expression in FDP-stimulated HDFs
3.3. EGCG inhibits the NF-kB activation, reduces AP-1 phosphorylation, and suppresses MAPKs activation in FDP-stimulated HDFs
4. Conclusion
Conflicts of interest
Acknowledgements
References
1. Introduction
2. Materials and methods
2.1. Chemicals and reagents
2.2. Cell culture
2.3. Determination of the damage of FDP-stimulated HDFs
2.4. Determination of cytotoxicity of EGCG on HDFs
2.5. Determination of the effect of EGCG against FDP-stimulated HDFs
2.6. Determination of relative intracellular elastase and collagenase activities
2.7. Determination of collagen synthesis and MMPs expression levels by ELISA
2.8. Western blot analysis
2.9. Statistical analysis
3. Results and discussion
3.1. FDP causes oxidative damage and EGCG improves FDP-induced oxidative damage in HDFs
3.2. EGCG inhibits intracellular enzymes activities, protects collagen synthesis, and reduces MMPs expression in FDP-stimulated HDFs
3.3. EGCG inhibits the NF-kB activation, reduces AP-1 phosphorylation, and suppresses MAPKs activation in FDP-stimulated HDFs
4. Conclusion
Conflicts of interest
Acknowledgements
References
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