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Purpose: The purpose of this study was to identify the relationship between nursing error experience, coping behavior and job stress in operating room. Methods: A descriptive research design was used in this study. The participants were 228 operating room nurses in G city who surveyed between October 25 and November 25, 2017 using self-report questionnaires. The data were analyzed using IBM SPSS/WIN 24.0/AMOS WIN 24.0 Program, which determined frequency, percentage, mean, standard deviation, Pearson correlation coefficient, and structural equation model. Results: There were significant positive correlations between six sub-categories of nursing errors and job stress. We found negative correlations between coping behavior and job stress. There was a mediating effect of active coping between knowledge of nursing error and job stress. We found passive coping between inspection & monitoring related error and job stress. Conclusion: Study findings suggest that adequate education and the improvement in hospital environment and system should be required to reduce the nurses’ job stress related to the patients’ safety in operating room.