원문정보
초록
영어
Pleurotus ostreatus No.42, known as the ligninolytic basidiomycetes, showed production of MnP and Lac, but did not show any LiP acitivity in static culture, grown in GPYW liquid medium. Maximum production of MnP (80U/flask) was observed on day 11 of culturing in this medium. Chromatographic purification of MnP included the use of Sepharose CL-6B and Mono-Q. The major MnP isozyme purified by column chromatography was observed to be a 36.4 KDa (single band on SDS PAGE). The 19-amino acid sequence from the N-terminal was determined by protein sequencing to be ATCADGRTTANAACCVLFP. The N-terminal sequence of the major MnP isozyme of P. ostreatus No.42 was found to be the same as a previously reported sequence of an MnP3 isozyme from this fungus.
목차
서 론
재료 및 방법
공시균주 및 배양방법
리그닌 분해효소 측정
조효소액 준비 및 농축
총 단백질 함량
효소의 정제
겔 전기영동
N-말단 아미노산 서열분석
결과 및 고찰
글루코오스-펩톤-이스트-밀기울 배지(GPYW)에서 정치배양한 리그닌분해효소 생산
망간퍼옥시데이즈 분리 및 정제
겔 전기영동
N- 말단 아미노산 분석
적 요
REFERENCES