원문정보
초록
영어
There is a strong connection between the diet rich in antioxidants and the decreased incidence of cardiovascular and cancerous diseases. Diets that are rich in anti-oxidants particularly include fruits and vegetables containing the high amounts of vitamin A-E, carotenoids, and minerals. Different processing conditions applied for vegetables and plants results in the alteration of the nutrients present in them. Therefore the rationale of our study was to compare the antioxidant effects of different processed vegetables and plants and to see that which one of them showed best anti-oxidant activity. For this purpose, we have used acetaminophen induced oxidative stress model in mice to check the effects of processed apple, pear, carrot, cabbage, broccoli and radish. Our results have shown that the administration of these samples effectively decreased the expression of parameters related with oxidative stress like ALT, AST, catalase, superoxide dismutase, GPx and 8-OHdG. Moreover they also significantly protected the mice livers from APAP induced damage as shown by histological changes. Therefore our results have demonstrated the effects of processed fruits and vegetables in mice model of oxidative stress.
목차
1. INTRODUCTION
2. EXPERIMENT MATERIALS AND METHODS
2.1. Preparation of Processed fruits and vegetables Extract
2.2. Experimental animals and sample administration
2.3. Assessment of serum 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels
2.4. Preparation of liver homogenate
2.5. Measurement of antioxidant enzyme levels in liver tissue
2.6. Evaluation of liver damage by serum biochemical analysis
2.7. Hematoxylin & Eosin (H&E) staining for liver tissueThe liver tissue was harvested from mice after euthanasia and
2.8. Statistical analysis
3. RESULT AND DISCUSSION
3.1. Amelioration in the activity of ALT and AST by fruits and vegetables samples:
3.2. Restoration of the levels of SOD, GPx and catalase activity by fruits and vegetables samples:
3.3. Reduction in the levels 8-OHdG by fruits and vegetables samples
3.4. Effects of fruits and vegetables samples on APAP induced hepatic damage
5. CONCLUSION
ACKNOWLEDGMENTS
REFERENCES