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Objectives: This study examined the degree of professional autonomy of nurses as perceived by nurses and doctors, as well as the relation between professional autonomy, job satisfaction, and turnover intention of nurses. Methods: A questionnaire survey was conducted with 292 nurses and 121 physicians working in 10 small- and medium-sized hospitals with located in G Metropolitan City. Results: The difference in the level of nurses’ professional autonomy as perceived by nurses and physicians was found to be significantly higher in nurses than in physicians. Professional autonomy and job satisfaction were significantly and positively correlated; professional autonomy and turnover intention were significantly and negatively correlated; and job satisfaction and turnover intention were also significantly and negatively correlated. Conclusions: Various efforts to increase nurses’ professional autonomy and the development of effective workforce management programs to prevent turnover should be actively conducted to address the problems of small- and medium-sized hospitals facing a shortage of nurses.