earticle

영어

In recent years, there has been an increasing number of people taking hemp seeds (HS), especially imported HS, instead of nut for health care purposes in Korea. In addition, there is a growing awareness of whether delta 9-tetrahydrocannabinol (THC), a hallucinogenic component of cannabis, can be detected in urine when taking commercially available HS products. Thus, it is important to determine whether delta 9-tetrahydrocannabinol carboxylic acid (THC-COOH) is detected in the urine of people taking commercially available HS products and, if detected, the concentration of THC-COOH detected in urine 2 - samples of cannabis abusers and HS product onsumers, it is necessary to review.Thus, in this study, we analyzed the content of THC in the HS by purchasing two HS products (HS1 & HS2) in market. In addition, 13 volunteers were recruited, 6 for male and 7 for female. HS1(THC low level contained product, 1.58 mg/g) and HS2(THC high level contained product, 5.18 mg/g) were administered to each 3 male and female volunteers once a day for 28 days at a dose of 30 g, respectively. And one female administered same dose of mixed nuts (MN) for control. Urine samples of 13 volunteers were collected on the day before taking the HS or MN, once every week for 28 days. For THC analysis in HS, 1g of HS was added to THC-d3 as an internal standard and methanol, and extracted using ultrasonic device for 2 hour. This process was repeated three times. The COBAS C311 was used to screen the urine samples, and GC/MS was used to determine the amount of THC-COOH. THC-COOH levels in volunteers’urine ranged from 0 to 11 ng/mL. During the volunteers’ 28-day period, all of the THC-COOH were under the cutoff level. Therefore, this study is expected to be a good answer and basic study on the questions related to the consumption of HS products, as well as establishing the criteria for detection of THC-COOH in the urine of consumers of HS and cannabis abusers.