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Miscanthus is a perennial rhizomatous grass originated in the tropics and subtropics although different species are adapted to be found throughout a wide climatic range worldwide including Asia. M. giganteus is direct planting of rhizomes into the field (referred to in the following sections as ‘rhizome planting’) in general. It means it has to be stored for long term in the low temperature for cultivation for large field cultivation. Further, to manage plants well, it is important to have uniform germination. However, the germination rate of Miscanthus species depending on the rhizome size was not studied well. Thus, it would be crucial to know the effect of long-term storage in the low temperature for the germination of rhizome. Here, we tested rhizomes of M. giganteus with various storage periods in the low temperature to examine how the germination of rhizome changes. Also, we tested the germination rate and germination days depending on the rhizome size of three Miscanthus species including M. sinensis, M. sacchariflorus, and M. giganteus. Over all, storage period in 4-8 weeks performed very well compared to others and it would be good to use 15-20 g of rhizome for M. giganteus and M. sinensis for 100 % germination.