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From the Botanical Garden of the Faculty of Science, Charles University and Prague Botanical Garden in Czech Republic, 175 plants were examined by photographing. 157 species in 44 families of plants were investigated in the Botanical Garden of Charles University, Among this botanical garden, the most dominant family was Compositae with 31 species, followed by Lamiaceae with 25 species, Apiaceae with 13 species, Solanaceae with 9 species, and Leguminosae with 7 species. Ferula assa-foetida L., Ammi visnaga (L.) Lam., Trigonella foenum-graecum L., Tussilago farfara L., and Levisticum officinale W.D.J.Koch are important medicinal plants grown in the Botanical Garden of Charles University. From the greenhouses of Prague Botanical Garden, 18 species in 12 families of plants were classified. Commiphora simplicifolia Schweinf. is an important plant in this botanical garden.