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논문검색

생맥산의 구성 생약 중 오미자의 schizandrin과 gomisin A의 정량 분석

원문정보

Quantitative Analysis of Schizandrin and Gomisin A in Traditional Herbal Formula, Saengmaek-san

서창섭, 김정훈, 신현규

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Saengmaek-san (SMS) comprises three herbal medicines Liriopis seu Ophiopogonis Tuber, Ginseng Radix, and Schisandrae Fructus. SMS has been traditionally used for the treatment of ischemic disease in Asia. In this study, an analytical method using high-performance liquid chromatography (HPLC) and a photodiode array detector (PDA) (HPLC PDA) was used for the quantitative analysis – of schizandrin and gomisin A in SMS, a traditional herbal formula. Chromatographic separation of the two compounds was carried out using a Gemini C18 column and two mobile phases, distilled water and acetonitrile, with gradient elution. The coefficient of determination of the calibration curve for the quantitative analysis of the marker components of Schisandrae Fructus in an SMS sample, schizandrin and gomisin A, was 1.0000. The limit of detection of the marker components was 3.28 mg/mL and 3.19 mg/mL, and the limit of quantification was 9.93 mg/mL and 9.67 mg/mL. Recovery and intra- and interday precisions were 95.44-103.58% and <1.5%, respectively. The quantities of schizandrin and gomisin A were 0.244 and 0.028 mg/freeze-dried g, respectively. The optimized HPLC–PDA method could be expected to provide basic data for the quality control of SMS and other related herbal formulas.

목차

Abstract
Introduction
Discussion
1) Materials and methods
2) Results and discussion
Conclusion
Acknowledgments
References

저자정보

  • 서창섭 Chang-Seob Seo. 한국한의학연구원 한약연구부
  • 김정훈 Jung-Hoon Kim. 한국한의학연구원 한약연구부, 부산대학교 한의학전문대학원 약물의학부
  • 신현규 Hyeun-Kyoo Shin. 한국한의학연구원 한약연구부

참고문헌

자료제공 : 네이버학술정보

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