earticle

영어

Simple sequence repeats (SSRs) were isolated from the genome sequences of “Heuktari” and “Miso” those were sequenced by Pac-Bio sequencing technology. The total numbers of SSRs detected in the “Heuktari” and “Miso” genomes were 949 and 968, respectively. These SSR numbers the highest among five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii. These are 133% and 135% of PC9 and PC15, and 145% of P. eryngii, respectively. The similar pattern of SSR distributions were found in all mushrooms, which Tri-, hexa- and octanucleotide motifs accounted for the top three fractions of SSR distributions. These SSRs could be used as a primer for genotyping of segregation population and screening for the trait linked markers. In SSR distribution patterns, all mushrooms studied showed similar results. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs. Over Three repeats or more of 10-mers accounts for 9.8 in “Heuktari” and 6.5% in “Miso”. [Supported by a grant from the IPET (213003-04-3-SBY20), MIFAFF, Republic of Korea.]