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Original Article

Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells

원문정보

Byeong Mun Kwak, Ee-Hwa Kim, Yong-Min Kim, Hee-Tack Kim

한국운동재활학회 JER Vol.15 No.5 2019.10 pp.723-730
피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The main purpose of this study was to suggest a solution for inflamma-tion caused by urban daily life based on supercritical extraction of Cha-maecyparis obtusa Endl. (C. obtusa). The leaves, cone, wood, and bark of C. obtusa were separately extracted with supercritical extraction. Al-pha-pinene, anti-inflammatory active compound of C. obtusa, was ana-lyzed by electrospray ionization-mass spectrometry, gas chromatogra-phy, and gas chromatography mass spectrometry from each part of ex-tracts. As a result of the reduction rate of nitric oxide in RAW 264.7 cells, the wood extract exhibited the highest reduction rate was about 45% among extracts from four part of C. obtusa. The wood extract from C. obtusa can be developed as a good anti-inflammatory natural raw ma-terial for natural product industry.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Supercritical fluid extraction
Fatty acid analysis
Electrospray ionization-mass spectrometry
Solid phase microextraction method
Nitric oxide assay
RESULTS
Supercritical fluid extraction
Fatty acid component
GC/MS analysis
Effects of extracts from C. obtusa and α-pinene on NO
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

저자정보

  • Byeong Mun Kwak Department of Meridian & Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea
  • Ee-Hwa Kim Department of Meridian & Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea
  • Yong-Min Kim Department of Oriental Medical and Herbal Cosmetic Sciences, Semyung University, Jecheon, Korea
  • Hee-Tack Kim Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University, Jecheon, Korea

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