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Purpose: This study aimed to assess college students’ knowledge, attitude, and health behavior regarding cancer based on 10 national cancer prevention recommendations and to examine the relation between these variables. Methods: The participants were 189 college students in J city. Data were collected with a questionnaire consisting of items on cancer-related knowledge, attitude toward cancer, and implementation of 10 national recommendations on cancer. The data were analyzed using independent two-sample t-test, one-way analysis of variance, Scheffé multiple comparison test, Pearson’s correlation coefficients, and multiple regression(stepwise) analysis with SPSS/WIN 21.0 program (IBM, Armonk, NY, USA). Results: The mean scores for knowledge, attitude, and health behavior were 23.83 points (out of a possible 32), 33.10 points (out of possible 50), respectively. Attitude was positively correlated with health behavior and accounted for 37.7% of cancer prevention health behavior. Conclusion: The findings of this study suggest that the related factors should be considered in the development and implementation of systemic education programs that can encourage and promote cancer prevention health behavior among college students.