The purpose of this study was to determine the effects of combined exercise training on body composition, physical fitness, and blood lipids in middle-aged obese women. Twenty-one middle-aged obese women (age, 55.28 ± 5.95; body fat percentage, 36.88 ± 4.29) were recruited and conducted combined exercise training for 12 weeks. To determine the effects of combined exercise training, we measured the body composition, physical fitness, and blood lipids before and after combined exercise training (aerobic exercise + resistance exercise, 3 times a week for 12 weeks). Combined exercise training for 12 weeks improved body composition, including body weight, body mass index, body fat percentage, and visceral fat percentage. In addition, combined exercise training attenuated blood lipid profiles, including blood glucose, high-density lipoprotein-cholesterol (HDL-C), low-density lipoproteincholesterol (LDL-C), and total cholesterol (TC). In contrast, physical fitness factors, including muscle strength, muscle endurance, and flexibility were significantly improved by combined exercise training for 12 weeks. We demonstrated that combined exercise training attenuated obesity-induced alteration of body composition and blood lipid profiles, and improved physical fitness in middle-aged women, suggesting that combined exercise training plays an essential role in protecting against obese-induced alteration of body composition, physical fitness, and blood lipids in middle-aged obese women.