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논문

Characteristics and Anti-bacterial Effects of Mineral Supplement-Hypochlorous Acid Water on Human Pathogenic Bacteria

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초록

영어

Hypochlorous acid (HOCl) is a powerful oxidant and a bactericidal agent generally produced by electrolysis, which is much more effective and safer than NaOCl. Recently mineral supplement-treated HOCL (MS-HOCl) water has received attention as a new-concept detergent due to high sterilization power, easy accessibility and safety. In this study we time-dependently investigated the physiochemical properties of MS-HOCl water depending on the kind of diluting agents (tap water, low concentration mineral water and high concentration mineral water), and confirmed anti-bacterial effect of MS-HOCl water according to the ACC level compared to electrolyzed acidic water. Weak acid electrolyzed water (EW-HOCl) was used as a positive control. We evaluated the pH, oxidation reduction potential (ORP), available chlorine concentration (ACC) and total dissolved solid particles (TDS) level to detect physiochemical properties of various kind of MS-HOCl waters. To investigate the bactericidal effects of these experimental waters, 5 different strains of aerobic and anaerobic human pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans and Helicobacter pylori) were used. Three MS-HOCl waters according to ACC levels were prepared through 2-fold serial dilution, and compared with tap water, 70% ethyl alcohol and EW-HOCl. In results, MS-HOCl water had similar physiochemical properties compared to EW-HOCl water. Moreover, MS-HOCl treatment showed excellent bactericidal effect against all five human pathogenic bacterial strains compared to TW and 70% alcohol, and showed similar anti-baterial effect with EW-HOCl. Collectively, our study indicates that MS-HOCl water could be used as a efficient disinfectant alternative due to cost-effectiveness and easy accessibility in hospital settings, food process and poultry industries. However, further studies are required for its safety use.

목차

ABSTRACT
Introduction
Materials and Methods
1. Preparation of MS-HOCl water and acidic electrolyzed water (EW-HOCl),and time-dependent measurement of ACC
2. Evaluation of physiochemical properties of MS-HOCl solution according to the kind of diluting agent.
3. Evaluation of anti-bacterial effect of MS-HOCl according to the ACC level
4. Statistical Analysis
Results and Discussion
Acknowledgement
Conflict of interest
References

저자정보

  • Md. Habibur Rahman Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Yonsei University Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea
  • Johny Bajgai Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Yonsei University Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea
  • Ailyn Fadriquela Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea, Department of Global Medical Science, Yonsei University Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea
  • Ailyn Fadriquela Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea
  • Kyu-Jae Lee Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea, Institute for Poverty Alleviation and International Development, Yonsei University, Wonju, Gangwon 26493, Republic of Korea

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