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Objectives: The current study intended to find the awareness and compliance with the standard precautions of the laboratory technicians working in the hospitals in Gyeonggi Province. Understanding on the relationships among factors would be helpful in developing work environment of the laboratory technicians to prevent infection and improving the quality of infection control in hospitals. Methods: Data were collected from the 313 laboratory technicians in hospitals in Gyeonggi Province. SPSS 21.0. and AMOS 18 were adopted to analyze the data. Results: The results of the analysis confirmed that the influence of perceived sensitivity and cues to action influenced perceived threats of disease. Perceived threats and perceived barriers to compliance with the standard precautions and perceived benefits of compliance with the standard precautions significantly influenced the probability of compliance with the standard precautions. Conclusion: It was recommended that hospitals need to prepare plans to prevent the employees from infection in the hospitals. The plans must include education, regular exams, injury prevention, and provision of personal protective equipments.