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Purpose: The purpose of this study was to investigate whether the respiratory function of patients with stroke was different on the ground and under water. Methods: We recruited 14 adults who had experienced a stroke (12 male, 2 female) for our study. We measured forced vital capacity, forced expiratory volume at one second, maximum inspiratory pressure, and maximum expiratory pressure when the participants breathed on the ground and under water. On the ground, the participants were safely supported using a table and chair and were measured in a standing posture. For measuring under water, the participants were immersed in water in a standing position to clavicle height. The participants were measured while standing, and the assistant supported them when they needed help. The collected data were analyzed by a paired t-test. Results: Forced vital capacity and forced expiratory volume at one second were significantly lower in water than on the ground when breathing at maximum. Maximum inspiratory pressure was not significantly different when standing on the ground or in water, but maximum expiratory pressure was significantly higher in water than on the ground. Conclusion: It has been confirmed that the hydrostatic pressure affecting stroke patients immersed in water affects the forced expiratory volume at one second while reducing the forced vital capacity and increasing the maximum expiratory pressure.