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논문검색

Hair Growth Promoting Effect of Radish Crude Saponin Extract on Athymic Nude Mice

원문정보

초록

영어

This study investigates the hair restoration efficacy of selected radish saponin extracts on nude mice. Nude mice genetically predisposed to pattern balding were used in this study. Our study revealed the underlying mechanism of stimulating hair growth in athymic nude mice by repair the nu/nu follicular keratin differentiation defect. Thus, the topical application of radish saponin may represent a novel strategy for the management and therapy of certain forms of alopecia. The term of hair density of PEE treated nude mice were significantly increase as compared with of control nude mice. Histological observation of skin sample showed no hair follicle or only distorted hair follicles were observed in the control samples, in contrast, by the PEE treatment groups showed a fully formed and increased the number of hair follicles up to three times higher than that of control group in terms of the number of hair follicles in nude mouse skin.PEE treated mice the number of BrdU-labeled keratinocytes per anagen follicle increased significantly, especially in the follicular bulbs and outer root sheath compared with the control mice. Moreover, PEE-treated nude mice also exhibited a significant increase in the number of BrdU-labeled epidermal keratinocyte proliferation.

목차

Abstract
1. Introduction
2. Experiment Materials and Methods
2.1 Heat Treatment of Radish and Preparation of Extract
2.2 Extraction and Fractionation of Radish saponin
2.3 Experimental animals
2.4 Administration of PEE and fractions of Radish crude saponin
2.5 Evaluation of hair coverage area
2.6 Evaluation of hair density
2.7 Histologic assessment of hair growth
2.8 Assessment of Keratinocyte Proliferation with Anti-BrdU
2.9 Statistical analysis
3. Result and Discussion
3.1 Macroscopic observation of hair changing pattern on nude mice:
3.2 Evaluation of hair coverage area
3.3 Determination of hair density
3.4 Histological investigation of the skin of nude mouse
3.5 Number of hair follicle
3.6 Stimulates Proliferation of Follicular and Sebaceous Gland Keratinocytes
4. Conclusion
References

저자정보

  • Hyun-Kyoung Kim Department of Food Science and Engineering, Seowon University, Cheongju, Korea

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